Production of a Cold-Active Lipase by Fusarium Solani

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Published: Sep 15, 2024
Updated: 2024-09-15
Keywords:
cold active enzymes, Lipase, Fusarium solani, lipolytic activity, specific activity

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Joheni M Jwely
University of Zawia
Mohamed A Al-Ryani
Zintan University

Abstract

The current study aimed to the production and partial purification of a cold-active lipase by some fungi isolated from the olive oil processing wastes in Al-Gabal Al-Gharby, Libya. 31 fungal species from 12 genera were isolated. F. solani was the most prevalent comprising 94% of total Fusarium and 28.7% of total fungi, 102 fungal isolates were tested for their lipolytic activity on lipase production agar medium at 10 and 20°C. The most active isolates were Alternaria (2 isolates), Fusarium, and Penicillium (1isolate for each one). Molecular identification of the most active four isolates was carried out by their sequencing (ITS). The four powerful fungal strains' production of cold-active lipase was maximized by optimizing some nutritional and environmental factors. F. solani AUMC 16063 was able to produce the maximum amount of lipase activity(46.66U/mL/min) with specific activity(202.8U/mg), utilizing ammonium sulphate as a nitrogen source after 8 days of incubation at pH 3.0 and 15°C. However, at same condition after 6 days when yeast extract was employed as a nitrogen source, the generated cold-active lipase displayed the highest specific activity of (1550U/mg) and lipase activity (36.74U/ml/min). This is the first study in which the production, partial purification, maximized and characterization of a cold-active lipase enzyme by Fusarium solani.

Article Details

Issue
Vol. 1 No. 1 (2024): Univ Zawia J Nat Sci
Section
Botany

References

Liu, X., Kokare, C., (2023). Microbial enzymes of use in industry. Biotechnology of microbial enzymes. Elsevier, pp. 405-444.

Kumar, A., Verma, V., Dubey, V.K., Srivastava, A., Garg, S.K., Singh, V.P., Arora, P.K., (2023). Industrial applications of fungal lipases: A review. Frontiers in Microbiology 14, 1142536.

Geoffry, K.,and Achur, R.N., (2018). Screening and production of lipase from fungal organisms. Biocatalysis and agricultural biotechnology14, 241-253.

Patel, G.,and Shah, K., (2020). Isolation, screening and identification of lipase producing fungi from cotton seed soapstock. Indian Journal of Science and Technology13 (36), 3762-3771.

Ali, S., Khan, S.A., Hamayun, M., Lee, I. J., (2023). The recent advances in the utility of microbial lipases: A review. Microorganisms11 (2), 510.

Basheer, S.M., Chellappan, S., Beena, P., Sukumaran, R., Elyas, K., Chandrasekaran, M., (2011). Lipase from marine Aspergillus awamori btmfw032: Production, partial purification and application in oil effluent treatment. New Biotechnology28 (6), 627-638.

Carvajal Barriga, E.J., and Portero Barahona, P., (2019). Production, extraction and characterization of lipases from the antarctic yeast Guehomyces pullulans.

Lima, L.G.R., Gonçalves, M.M.M., Couri, S., Melo, V.F., Sant’ana, G.C.F., Costa, A.C.a.D., (2019). Lipase production by Aspergillus niger by submerged fermentation. Brazilian Archives of Biology and Technology62.

Gupta, N., Sahai, V., Gupta, R., (2007). Alkaline lipase from a novel strain Burkholderia multivorans: Statistical medium optimization and production in a bioreactor. Process Biochemistry42 (4), 518-526.

Grbavčić, S., Dimitrijević-Branković, S., Bezbradica, D., Šiler-Marinković, S., Knežević, Z., (2007). Effect of fermentation conditions on lipase production by Candida utilis. Journal of the Serbian Chemical Society72 (8-9), 757-765.

.[11]Khan, F.I., Lan, D., Durrani, R., Huan, W., Zhao, Z., Wang, Y., (2017). The lid domain in lipases: Structural and functional determinant of enzymatic properties. Frontiers in bioengineering and biotechnology 5, 16.

Mehta, A., Bodh, U., Gupta, R., (2017a). Fungal lipases: A review. Journal of Biotech Research8.

Tan, J.S., Abbasiliasi, S., Ariff, A.B., Ng, H.S., Bakar, M.H.A., Chow, Y.H., (2018). Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system. 3 Biotech8, 1-7.

Bharathi, D., and Rajalakshmi, G., (2019). Microbial lipases: An overview of screening, production and purification. Biocatalysis and Agricultural Biotechnology 22, 101368.

Tan, T., Zhang, M., Wang, B., Ying, C., Deng, L., (2003). Screening of high lipase producing Candida sp. And production of lipase by fermentation. Process Biochemistry39 (4), 459-465.

Chandra, P., Enespa, Singh, R., Arora, P.K., (2020). Microbial lipases and their industrial applications: A comprehensive review. Microbial cell factories19, 1-42.

Cai, Y., Wang, L., Liao, X., Ding, Y., Sun, J., (2009). Purification and partial characterization of two new cold-adapted lipases from mesophilic Geotrichum sp. Sybc wu-3. Process Biochemistry44 (7), 786-790.

Abada, E. M., (2008). Production and characterization of a mesophilic lipase isolated from Bacillus stearothermophilus ab-1. Pakistan Journal of Biological Sciences11 (8), 1100-1106.

Dieckelmann, M., Johnson, L., Beacham, I., (1998). The diversity of lipases from psychrotrophic strains of Pseudomonas: A novel lipase from a highly lipolytic strain of Pseudomonas fluorescens. Journal of Applied Microbiology85 (3), 527-536.

Zeng, X., Xiao, X., Wang, P., Wang, R., (2004). Screening and characterization of psychrotrophic, lipolytic bacteria from deep-sea sediments. Journal of microbiology and biotechnology14 (5), 952-958.

Dominguez De Maria, P., Carboni-Oerlemans, C., Tuin, B., Bargeman, G., Van Der Meer, A., Van Gemert, R., (2005). Biotechnological applications of Candida antarctica lipase a: State-of-the-art. Journal of molecular catalysis. B: Enzymatic37 (1-6), 36-46.

Romo-Sánchez, S., Alves-Baffi, M., Arévalo-Villena, M., Úbeda-Iranzo, J., Briones-Pérez, A., (2010). Yeast biodiversity from oleic ecosystems: Study of their biotechnological properties. Food microbiology27 (4), 487-492.

Warcup, J., (1950). The soil-plate method for isolation of fungi from soil. Nature166 (4211), 117-118.

Ellis, M., (1976). More dematiaceous hyphomycetes (p. 507). Kew, Surrey, England: Commonwealth Mycological Institute.

Pitt, J.I., (1979). The genus Penicillium and its teleomorphic states Eupenicillium and talaromyces. Academic Press.

Raper, K.B., and Fennell, D.I., (1965). The genus Aspergillus. Williams and Wilkins, Baltimore, U.S. A.

Booth, C., (1971). The genus Fusarium commonwealth mycological institute. Kew, Surrey237.

Leslie, J., and Summerell, B., (2006). The Fusarium laboratory manual blackwell publishing. Ames, Iowa.

Moubasher, A., (1993). Soil fungi in Qatar and other Arab countries The Centre for Scientific and Applied Research, University of Qatar.

Domsch, K., Gams, W., Anderson, T., (2007). Compendium of soil fungi. 1–672. IHW-Verlag, Eching, Germany.

Ullman, U., and Blasins, G., (1974). A simple medium for the detection of different lipolytic activity of microorganisms. Zbl. Bakt. Hyg., II Abt. Orig. A229, 264-267.

Moharram, A.M., Zohri, A.-N.A., Hesham, A.E.-L., Abdel-Raheam, H.E., Al-Ameen Maher, M., Al-Bedak, O.a.-H., (2022). Production of cold-active pectinases by three novel Cladosporium species isolated from egypt and application of the most active enzyme. Scientific Reports12 (1), 1-17.

Mayordomo, I., Randez-Gil, F., Prieto, J.A., (2000). Isolation, purification, and characterization of a cold-active lipase from Aspergillus nidulans. Journal of Agricultural and Food Chemistry48 (1), 105-109.

Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., (1951). Protein measurement with the folin phenol reagent. Journal of biological chemistry193, 265-275.

Joseph, B., Upadhyaya, S., Ramteke, P., (2011). Production of cold-active bacterial lipases through semisolid state fermentation using oil cakes. Enzyme research2011.

Al-Bedak, O.a.-H.M., Moharram, A.M., Hussein, N.a.-G., Taha, D.M., Stephenson, S.L., Ameen, F., (2023). Microbial exploitation of feather wastes for sustainable production of keratinase and collagenase enzymes by Didymella keratinophila AUMC 15399 in submerged fermentation. Fermentation9 (6), 507.

Ji, X., Li, S., Wang, B., Zhang, Q., Lin, L., Dong, Z., Wei, Y., (2015). Expression, purification and characterization of a functional, recombinant, cold-active lipase (lipa) from psychrotrophic Yersinia enterocolitica. Protein Expression and Purification115, 125-131.

Dalmau, E., Montesinos, J., Lotti, M., Casas, C., 2000. Effect of different carbon sources on lipase production by Candida rugosa. Enzyme and microbial technology26 (9-10), 657-663.

Stahle, L., Wold, S., (1989). Analysis of variance (anova). Chemometrics and intelligent laboratory systems 6 (4), 259-272.

Mase, T., Matsumiya, Y., Akiba, T., (1995). Purification and characterization of a new lipase from Fusarium sp. Ym-30. Bioscience, biotechnology, and biochemistry 59 (9), 1771-1772.

Knight, K., Pimentel, M.D.C.B., Morais, M.M.C.D., Ledingham, W.M., Lima Filho, J.L.D., Maia, M.D., (2000). Immobilization of lipase from Fusarium solani fs1. Brazilian journal of microbiology 31, 219-221.

Quayson, E., Amoah, J., Hama, S., Kondo, A., Ogino, C., (2020a). Immobilized lipases for biodiesel production: Current and future greening opportunities. Renewable and Sustainable Energy Reviews134, 110355.

Rifaat, H.M., El-Mahalawy, A.A., El-Menofy, H.A., Donia, S.A., (2010). Production, optimization and partial purification of lipase from Fusarium oxysporum. Journal of Applied Sciences in Environmental Sanitation5 (1).

Borges, J.P., Quilles Junior, J.C., Ohe, T.H.K., Ferrarezi, A.L., Nunes, C.D.C.C., Boscolo, M., Gomes, E., Bocchini, D.A., Da Silva, R., (2021). Free and substrate-immobilised lipases from Fusarium verticillioides p24 as a biocatalyst for hydrolysis and transesterification reactions. Applied Biochemistry and Biotechnology193, 33-51.

Rashid, N., Shimada, Y., Ezaki, S., Atomi, H., Imanaka, T., (2001). Low-temperature lipase from psychrotrophic Pseudomonas sp. Strain kb700a. Applied and Environmental Microbiology67 (9), 4064-4069.

Choo, D.-W., Kurihara, T., Suzuki, T., Soda, K., Esaki, N., (1998). A cold-adapted lipase of an alaskan psychrotroph, Pseudomonas sp. Strain b11-1: Gene cloning and enzyme purification and characterization. Applied and Environmental Microbiology64 (2), 486-491.

Suzuki, T., Nakayama, T., Kurihara, T., Nishino, T., Esaki, N., (2001). Cold-active lipolytic activity of psychrotrophic Acinetobacter sp. Strain no. 6. Journal of bioscience and bioengineering92 (2), 144-148.

Lee, H.-K., Ahn, M.-J., Kwak, S.-H., Song, W.-H., Jeong, B.-C., (2003). Purification and characterization of cold active lipase from psychrotrophic Aeromonas sp. Lpb 4. Journal of microbiology41 (1), 22-27.

Xiao, X., Wang, P., Wang, F., (2004). Screening and characterization of psychrotrophic, lipolytic bacteria from deep-sea sediments. Journal of microbiology and biotechnology 14 (5), 952-958.

Arpigny, J.L., Feller, G., Gerday, C., (1993). Cloning, sequence and structural features of a lipase from the antarctic facultative psychrophile Psychrobacter immobilis b10. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression1171 (3), 331-333.

Joseph, B., Ramteke, P.W., Thomas, G., 2008. Cold active microbial lipases: Some hot issues and recent developments. Biotechnology advances26 (5), 457-470.

Gupta, R., Gupta, N., Rathi, P., 2004. Bacterial lipases: An overview of production, purification and biochemical properties. Applied microbiology and biotechnology64, 763-781.